Vx = volume of dye used for sample solution titration (ml)
Vb = volume of dye used for blank titration (ml)
F = mg ascorbic acid equivalent to 1.0 ml indophenol standard solution
V3 =volume of sample solution (ss) taken to analyse (2 ml)
Vss =volume of sample solution (ml)
Ws =weight of edible part of samples used for extraction (g)
100 = conversion factor for converting 1 g to 100 g