Buckwheat is considered as a healthy cereal food, and it is essential to cultivate new buckwheat lines with goodstarch physicochemical properties for both consumers and food producers. Six novel buckwheat (Duoku, Dk)were generated by crossing of Golden buckwheat and Tatary buckwheat, and their kernel appearance propertiesand starch physicochemical properties were analyzed together with one domestic line (Cimiqiao) and one wildline (Yeku). The results showed that Dk samples had better appearance properties than two control samples. TheDk samples showed lower amylose content, similar amylopectin molecular structure and chain length distributions, and larger starch granules compared with Cimiqiao. The digestion results showed that two Dk samples:Dk6 & Dk9 had high resistant starch content; while the other two Dk samples: Dk37 & Dk38 had a steady glucosereleasing rate. The Dk samples also showed high gelatinization temperature, indicating they were good rawmaterials for producing glass noodle. This study proved that Dk buckwheat had unique starch physicochemicalproperties, and could be used as new food materials in the future.