The experimental design consisted of 4 treatments (3 different PNSB as biofertilizers and 1COF) and 2 controls as previously mentioned. The experiments were run in four replicates in each paddy field. Therefore, there were 24 plots located in the same field and the area of each plot was roughly 0.10 ha. The amount of each PNSB biofertilizer,the COF and carrier control applied was at 0.75 kg ha1 by sowing at various points in the plot to ensure good distribution