Detection of multiplex PCR against 5 target Vibrio sp was
performed at different cell numbers from oyster tissue homogenates
as shown in Fig. 3. When less than 103 cfu g-1 of the 5 Vibrio sp were
present in oyster, all the 5 species were not detected at the same
time. Since Bej et al. (1999) and Lee and Choi (1995) reported the
threshold cell number for the detection to be 103 cfu g-1, the
performance of the primer of the present study can be said to be
almost equivalent to that of the previous studies.