The functionalization of Ag3O4 nanoparticles and surface morphology further confirmed with HR-SEM with EDAX results. The
antimicrobial effect of the non-functionalized and functionalized Ag3O4 nanoparticles dispersed in water was investigated. Antimicrobial activities were evaluated against bacterial strain of Pseudomonas aeruginosa, Staphylococcus aureus, and fungi Aspergillus niger used in this work. The n-Ag3O4 nanoparticles exhibit excellent antibacterial and antifungal effect compared to f-Ag3O4 nanoparticles and AMB. The decreased effect of f-Ag3O4 nanoparticles is might be due to increase in particle size and the interaction of silver oxide nanoparticles with bacteria membrane and intracellular proteins decreases with functionalization.