REAGENT PREPARATION
Allow all the reagents to equilibrate to room temperature (20 – 25°C) prior to use. Return to
proper storage conditions immediately after use.
Wash buffer
Dilution buffer
Prepare dilution buffer by mixing 15 ml of the 10x dilution buffer with 135 ml of distilled or deionized water, which is sufficient for 1 x 96 tests. In case less volume is required, prepare the
desired volume of dilution buffer by diluting 1 part of the 10x dilution buffer with 9 parts of
distilled or de-ionized water. Concentrated dilution buffer may contain crystals. In case the
crystals do not disappear at room temperature within 1 hour, concentrated dilution buffer can
be warmed up to 37°C. Do not shake the solution.
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