1.5 mL of this ONC were transferred
to 270 mL of iron-supplements BMD 1% (11 g L?1 a-D(+)-glucose
monohydrate, 13.4 g L?1 YNB, 0.4 mg L?1 D(+)-biotin, 278 mg L?1
FeSO4?7H2O, 0.1 M potassium phosphate buffer, pH 6.0) per Ultra
Yield Flask and cultivated under the same conditions for approximately
60 h.