Fish were sampled using one of two techniques: the first method involved sampling fish live in the
sling, using a series of visible targets along the ventral surface of the
fish, where opercle bones meet, to insert a needle into the bulbus
arteriosis within 60 s of immobilization in the sling. Fish sampled live
in the sling were then either released back into the tank, without impact,
to be used for future experiments, orwere euthanized for additional
cardiac and other sampling studies.
Fish were sampled using one of two techniques: the first method involved sampling fish live in thesling, using a series of visible targets along the ventral surface of thefish, where opercle bones meet, to insert a needle into the bulbusarteriosis within 60 s of immobilization in the sling. Fish sampled livein the sling were then either released back into the tank, without impact,to be used for future experiments, orwere euthanized for additionalcardiac and other sampling studies.
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