The specificity of the primers (listed in Table 2) was validated
via PCR using genomic DNA from 44 yeast strains
(Table 1).The use of the ScHO primers produced a single
amplificon of c. 400 or 300 bp with species S. cerevisiae
and S. pastorianus, respectively. On the other hand, PCR
with the LgHO primers produced a single amplificon of
c. 700 bp only in the type strain of S. bayanus (Fig. 3).
No PCR product was detected from other related species
or groups with these primers.