Cyclic voltammetry has been regularly exploited to investigate the electrochemical interactions of mixed or pure cultures with an anode . These techniques can differentiate direct and indirect electron transfer process that occur within the anode-associated biofilms. Cyclic voltammograms have been used to interpret the electron transfer process occurring within an anode associated biofilm, which can vary depending on the stage of biofilm development, the biofilm community and power generation by the anode associated biofilm .