อาหารเลี้ยงเชื้อแบคทีเรีย was routinely maintained in Luria-Bertani (LB) broth (0.5% yeast extract, 1% NaCl, 1% tryptone) and incubated at 28 ± 2°C for 48 h to prepare a pre-culture with an
initial optical density of 0.6 at 600 nm. The strain was cultured on LB agar plant and in broth for both short- and long-term storage and subsequent use in experiments.