Approximately 10 g of ground sunflower seeds were placed in a thimble, which was inserted into a Soxhlet apparatus and
extracted with 200 mL of hexane (M/S = 1/20 g/mL). The extraction was performed at normal boiling point for 4 h and afterwards the solvent was evaporated until dryness. Obtained oil was stored at 20 C until further use.