Economically
wise, Posada et al. (2011) demonstrated that the most appropriate
downstream process is based on the BIOPOL flowsheet. This process
starts with a heat pretreatment stage at 85 C, followed by a
simultaneous chemical-enzymatic digestion with both NaOCl
(30% weight) and the pancreatin enzyme Burkholderia sp. PTU9
(2% weight), at 50 C and pH 9.0.