The marine bacteria strains S1, S2 and S3, were isolated on seawater culturing medium containing Hg2+,
Cd2+, Cr6+ or Ni2+ at concentrations of 20 mg L1 and more. The isolates showed tolerance to these heavy
metals. S1 grew in the presence of 120 mg L1 of Hg2+ and accumulated Hg2+ at pH 4–10. For the effect of
co-existing cations on bioaccumulation of target metal, it was found that the effect depended on not only
the variety and initial concentration of co-existing ions, but also the initial concentration of target metal
and biomass dosage. A new parameter, the ratio of remaining bioaccumulation capacity (RRBC) of biomass,
was therefore defined to evaluate such effect. Furthermore, the mechanism evaluation revealed
that S1 bound about 70% Hg2+ on the cell surface, and carboxyl group played an important role in Hg2+
binding.