Use of ooze and bark rubs from E. amylovora-infectedplants also showed E. amylovora detection, as indicatedby the fluorescence curves from BioRanger (Fig. 4). Wefurther noted that if samples from bacterial ooze werediscolored, or direct use of asymptomatic stem and leaves,did not cause a reaction. In contrast, rubbing the infectedbark to obtain bacterial suspensions provided successfulamplification of E. amylovora DNA and fire blight diagnosis(Fig. 4).