The E. coliO157:H7 andSalmonella
antibodies were specific to the organisms while theE.
coliantibody was reactive to allE. colistrains, including
E. coliO157:H7. The NC membrane, cellulose membrane, and fiber glass membrane grade G6 were
purchased from Millipore (Bedford, Massachusetts),
and the micro-tip silver pens were purchased from
Chemotronics (Kennesaw, GA). Nutrient broth (Difco
Laboratories, Detroit, MI) was used as the bacterial
culture media. Other reagents used were of analytical
grade. All chemicals and diluents were prepared with
doubly deionized water with conductivity below 0.1 mS/
cm.