The amount of nutrients required for the B treatment was calculated using the McCarthy method (Cookson, 1995) to obtain a C:N:P proportion of 100:8:1 (m/m/m), comparable with proportions applied in previous research (Atagana et al., 2003).Tween 80was applied to the soil at a final concentration of 6.55 mg/kg to ensure the micellar solubilization process,