2.3. Fabrication of protein-polymer hybrid scaffolds via electrospinning
PCL/collagen (75:25) was dissolved in HFIP at 6% total concentration (w/v). The solution was placed in a 1 mL syringe with a stainless steel needle (23.5 gauge), attached to a syringe pump (100 Series, Kd Scientific, USA) at a flow rate of 0.08 mL/h. A grounded copper plate was placed on 10 cm from the end of the needle and 14 kV of electrical potential was applied by high-voltage supplier (HV30, NanoNC, Korea) to generate the required potential difference between the tip of the needle to collector to produce fibers.