A high-performance liquid chromatograph
(Hitachi Ltd., Tokyo, Japan) equipped with an RI detector was used to analyze
the concentrations of glucose, xylose, xylitol, ethanol, and glycerol. A Bio-Rad
type HPX-87H ion-exclusion column was used. The mobile phase was 0.005 M
H2SO4 at a flow rate of 0.4 ml/min.