The leaves of transgenic plants were collected and used to check
the presence of Cry1Ac protein using an immunostrip assay
(Bt-Cry1Ab/1Ac ImmunoStrip
test following
the manufacturer’s instructions. The leaf sample (80–100 mg
per plant) was ground using liquid nitrogen and crude protein
was extracted using 1.5 ml 1sample extraction buffer 4 surface of the other leaf using a small brush. Then , the relative herbicide tolerance of the plants was evaluated visually one week after application.