Explants were cultured on MS basal medium supplemented with different concentrations and combinations of plant growth regulators, 6-Benzylaminopurine BAP: 1.0 - 20.0 µM), Kinetin (Kn:
2.5- 20.0 µM), 1-Naphthaleneacetic acid (NAA: 3.0- 20.0 µM) and B5 vitamins + 2mg / ltr. Glycine (MBG) formulation for shoot proliferation and multiplication. All cultures were incubated under
16h photoperiod with light intensity of 55μmolm–2s–2 provided by cool white fluorescent lamps (Phillips, India) at 25 ± 2°C. All the cultures were transferred to fresh medium after 2-3 week duration.
The frequency and number of shoots formed were evaluated after 6 weeks of inoculation. Morphological changes were recorded on the basis of visual observations at 3-week intervals