Chlorophyll and β-carotene (provitamin A) are natural pigments in higher plants that considered having the antioxidant properties. The structure of porphyrin in chlorophyll appears to be an essential part for the antioxidative activity and it seems that chlorophyll diminishes free radicals instead of decomposes the hydroperoxides whereas antioxidant capability of β-carotene is based on its singlet oxygen quenching characteristic [20]-[24]. Until now, there are various attentions in the study of antioxidants from callus of Ocimum . In sweet basil callus, the content of phenolic groups was investigated [10] while flavonoid and phenolic acid were mainly focused on callus of holy basil [11]-[13]. For lemon basil, the current outcomes pointed out that 2,4-D exactly had the influences on the amount of chlorophyll a, b and β-carotene. Higher concentration of 2,4-D treatment lessened those antioxidant components. This incident could probably occur as a result of unorganized chloroplast or less chlorophyll synthesis which evidently showed in Figure 6 (chloroplast was not found in most cells).
Chlorophyll and β-carotene (provitamin A) are natural pigments in higher plants that considered having the antioxidant properties. The structure of porphyrin in chlorophyll appears to be an essential part for the antioxidative activity and it seems that chlorophyll diminishes free radicals instead of decomposes the hydroperoxides whereas antioxidant capability of β-carotene is based on its singlet oxygen quenching characteristic [20]-[24]. Until now, there are various attentions in the study of antioxidants from callus of Ocimum . In sweet basil callus, the content of phenolic groups was investigated [10] while flavonoid and phenolic acid were mainly focused on callus of holy basil [11]-[13]. For lemon basil, the current outcomes pointed out that 2,4-D exactly had the influences on the amount of chlorophyll a, b and β-carotene. Higher concentration of 2,4-D treatment lessened those antioxidant components. This incident could probably occur as a result of unorganized chloroplast or less chlorophyll synthesis which evidently showed in Figure 6 (chloroplast was not found in most cells).
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