There was a good recovery of tocopherol from Ecoflex films (98–112%). Oleuropein and oleuroside were the main antioxidants detected in the studied olive leaf extract. A reduction of oleuropein content (21–33%) and an increase of oleuroside (14–31%) were observed in Ecoflex and Ecoflex/PLA films. All the films showed antioxidant capacity in vitro. The films containing tocopherol exhibited higher antioxidant activity than the films containing olive leaf extract.