noted the same effect when analyzing the
hydrolysis of sugarcane bagasse subjected to sulfuric acid pretreatment
or to acid–alkali pretreatment (sulfuric acid and sodium
hydroxide). In that study, the authors observed that after 24 h of
hydrolysis at 50 C using 7% (w/v) bagasse and 20 FPU of cellulases
per gram of substrate, approximately 10 and 20 g/L of glucose were
released with the acid pretreatment and the acid–alkali pretreatment,
respectively. In this work similar results were obtained with
the acid–alkali pretreatment using 15 FPU of enzyme and 8% (w/v)
of pretreated bagasse. The acid–alkali pretreatment was selected
for use in subsequent experiments because it showed the best
hydrolysis yield.