article info abstract
Article history:
Received 8 October 2015
Received in revised form 29 January 2016
Accepted 29 January 2016
Available online 26 February 2016
Synthesis of silver nanoparticles using microorganism are many, but there are only scanty reports using
actinobacteria. In the present study, the actinobacterium of the genus Sinomonaswas reported to synthesis silver
nanoparticles for the first time. A photo-irradiation based method was developed for the synthesis of silver
nanoparticles, which includes two day old cultural supernatant of novel species Sinomonas mesophila MPKL 26
and silver nitrate solution, exposed to sunlight. The preliminary synthesis of silver nanoparticles was noted by
the color change of the solution from colorless to brown; the synthesis was further confirmed using UV–visible
spectroscopy which shows a peak between 400 and 450 nm. Spherical shape silver nanoparticles of size range
4–50 nm were synthesized, which were characterized using transmission electron microscopy. The Fourier transform infrared spectroscopy result indicates that, the metabolite produced by the novel species S. mesophilaMPKL
26 was the probable reducing/capping agent involved in the synthesis of silver nanoparticles. The synthesized silver nanoparticles maintained consistent shape with respect to different time periods. The synthesized silver
nanoparticles were evaluated for the antimicrobial activity against multi drug resistant Staphylococcus aureus
which show good antimicrobial activity. The method developed for synthesis is easy, requires less time
(20 min) and produces spherical shape nanoparticles of size as small as 4 nm, having good antimicrobial activity.
Hence, our study enlarges the scope of actinobacteria for the rapid biosynthesis of silver nanoparticles and can be
used in formulating remedies for multi drug resistant S. aureus.
© 2016 Elsevier B.V. All rights reserved.
Keywords:
Sinomonas mesophila MPKL 26
Silver nanoparticles
Sunlight
Antimicrobial activity