The aqueous dried extract of EL, in the form of powder,
was obtained from the Department of Biochemistry,
Universiti Malaya, Malaysia. The extract was dissolved
in normal saline to a final concentration such that each rat
received EL at a dose of 8 mg/kg body weight in a volume
of 0.5 ml. Estradiol (Sigma Chemical Co, USA) was first
diluted using ethanol and olive oil (1:9 volume) to obtain the
desired concentration. The dispensing volume was 0.1 ml.