during which compound
precursor ions were detected by scanning from m/z 50 to 2000 with
column flow rate of 5µl/min solvent A consisted of 90% (water in
0.1% Trifluro acetic acid) and solvent B consisted of 10%
(acetonitrile in 0.1% Trifluro acetic acid) and 30 µl sample were
injected onto the column.