Rubber sheet wastewater was collected from a lagoon of a CRSF at Pichit suburb, Songkhla Province, Thailand. The collected wastewater was filtered through cheesecloth into a 25 liter non-transparent plastic tank and stored in a cold room at 6 ± 2°C until use. The wastewater without autoclaving was supplemented with 0.05% NH4Cl as a supplementary nitrogen source to allow a reasonable growth of PNSB (based on our preliminary work). This medium was named RAW since the non-sterile rubber sheet wastewater was used.