Image thresholding [4]-[6] is one of the cell segmentation methods used to segment the objects out of background
and it does not separate the touching nuclei. In cell images global threshold minimizes the variations between different
images which are important for separating the touching or overlapping nuclei but local threshold provides the most
suitable value for segmentation with better adaptability. Bin Fang et al. (2003) proposed a two-stage tumor cells
identification strategy [4]-[6], in its first stage it uses local adaptive threshold for automatic potential tumor cell
segmentation. Here, the global threshold [4]-[6] is very expensive and the clumped cells cannot be considered, because
it uses single threshold level for entire image. In global threshold, ‘brighter’ backgrounds are misclassified as cells and
‘darker’ cell regions are misclassified as background. By minimizing the negative effect of background noise, local
adaptive threshold segments the reg