10 μL of solution containing 1 mg/mL HRP, 1 mM MAOS, 10 mM 4-AAP, and oxidase was loaded onto the detection zones. GOx and LOx (10 mg/mL and 5 mg/mL, respectively) were immobilized on their corresponding detection zones. To transport the sample solution effectively, 8 μL PBST was used to pretreat the fluidic channel on the bottom layer