Three phages (VP-1, VP-2 and VP-3) were isolated from marine
water samples (salinity 18–21; pH 7.6–7.7) of a semi-intensive aquaculture
(earthen pond aquaculture system Corte das Freiras located in
the estuarine system Ria de Aveiro, latitude: 40°37′51.44″N, longitude
8°40′31.75″W, on the north-western coast of Portugal) using
V. parahaemolyticus as host. Five hundred milliliters of water were filtered
sequentially by 3 μm and then by 0.45 μm pore-size polycarbonate
membranes (Millipore, Billerica, USA). Filtered water was added to
500 mL of TSB with double concentration and to 1 mL of bacterial culture.
The mixture was incubated at 25 °C for 18 h at 80 rpm, and then