Sugar alcohol-producing strains are usually screened and quantified by thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC) and p-iodonitrotetrazolium violet (INT) methods [24–26]. However, these methods are time-consuming or suffer from high-cost and are limited for high throughput screening. Therefore, it is necessary to develop an
efficient screening approach for sugar alcohol-producing microbes.