According to these previous studies, the most suitable extraction
procedure for vitamin B1 and B2 in this kind of samples were
chosen through different acid and enzymatic hydrolysis (with
overnight incubation at 37 C) assays in order to separate both
vitamins from other compounds in the seeds (mainly proteins and
carbohydrates) (Table 1). Some extracts were concentrated after
incubation, using a Savant Speed Vac PD121P concentrator