Color development after the reaction with a phenol nitroprusside reagent and alkaline hypochlorite solution, was determined after incubation at 37 ◦C for 20 min and reading the absorbance at 635 nm.
Color development after the reactionwith a phenol nitroprusside reagent and alkaline hypochloritesolution, was determined after incubation at 37 ◦C for 20 min andreading the absorbance at 635 nm.