Female nematodes were dissected from the infected roots
and processed for morphological examination as outlined in
Shurtleff and Averre (2000). Male and juvenile specimens
were obtained from the infected roots by cutting the roots
into small pieces and incubating them at room temperature
(22 °C) for 48 h in a Petri dish half filled with distilled
water. Identifications were confirmed on specimens fixed
in formaldehyde for 2 weeks, then processed to glycerol
by the slow method and mounted on permanent slides
(Hooper 1986).