The Background Balance function will help reduce the effects of uneven illumination. To use it properly, please setup your microscope, focus on your slide and adjust the microscope's illumination and the Exposure settings above accordingly. Next move the slide away from the camera's Field of View and click on the "Background Balance" button.The screen will then be even. You can adjust exposure settings if you wish and also perform the White Balance function below. Next, move the slide back into the camera's field of view. Re-check the "Apply" checkbox of "Background Balance" to disable the corresponding function