Enumeration of aerobic mesophiles was
carried out on Plate Count Agar (PCA, Merck
5463, Darmstadt, Germany) and incubated at
30o
C for 3 days. The LAB micropopulation was
enumerated on deMan Rogosa and Sharpe medium
(MRS Oxoid) (deMan et al., 1960) after 10-fold
dilution of the sample and incubated anaerobically
in an anaerobic jar at 30o
C for 5 days. Yeasts were
enumerated on Oxytetracycline-GlucoseYeast
Extract Agar (OGYEA, Oxoid CM0545) containing
4 ml oxytetracycline (Oxytetracycline Selective
Supplement Oxoid) and incubated at 25o
C for 5 days.