Result from staining with Coomassie Blue from GelDoc in picture no. 1 and picture no. 2 found that rainbow in the first lane was low staining while sample was so intense. At the beginning, quantitation of protein of sample was low amount so we used protein without dilute that made the band of sample intense staining. Obviuously band protein that we can notice from the picture in the sample 2 there is low amount of protein weight about 25-40 kDa and present protein weight about 10 kDa .The sample no. 3 and 4 there are low amount of protein weight about 15 kDa. (in the middle of intense staining in lane sample no. 3 cause from print of container).The sample no. 7 there is predominate band in protein weight about 50-70 kDa more than the other.