In aseptic packaging systems, packaging materials are sterilized by various methods in order to kill microorganisms contained in the packages during forming and transport through the machine prior to filling. Experimental data as well as theoretical results from several years of research in the area of sterilization methods for effective inactivation of microorganisms on surfaces of aseptic packaging materials are compiled and presented in order to choose the right method of sterilization by the food processing industry for a successful operation. Hydrogen peroxide, with concentrations up to 30%, temperatures of up to 80°C and contact times up to 15 s, with or without wetting agent, has been found to be successful for inline aseptic packaging. The final product must not contain greater than 0.5 ppm H2O2. Economic considerations and non-uniform dose delivery to pre-formed containers inhibit commercial adoption of ionizing radiation sterilization in-line with aseptic packaging systems.