For the extraction of β-carotene, the oxidized sample of 200 mg was
dissolved in 2 mL of acetone and sonicated for 20 s. The samples were
than kept at −20 °C for 24 h. The TAGs crystallizes and β-carotene
moves to the acetonic fraction. The samples were then quickly filtered.
This method removes about 90% of the lipids. Saponification was
avoided in order to reduce the formation of artifacts (e.g. hydrolysis of
epoxides). For the analysis of TAGs and its oxidized species, a sample of
100 mg of corn oil was dissolved in 2 mL of acetone and 2 mL of HPLC
solvent. The sample was then transferred to a 2 mL vial for the HPLC
analysis.