Shoot Zn accumulation positively correlated with grain yield and grain Zn concentration. This relationship indicates that higher shoot Zn accumulation, resulting from greater N supply, may maximize grain yield and grain Zn concentration. The positive effects of N on root Zn uptake, root-to-shoot Zn translocation, and Zn accumulation in grain and shoot are of significance for Zn biofortification strategies in cereals and improving grain yields.