The infrared spectrum showed that the F12 which is the most active fraction has the functional groups of
hydroxyl, aromatics and C–O–C. It means that this fraction is consists of flavonol group compounds.
Phytochemical test analysis showed that F12 is consisted of flavonol. Flavonol compounds reported to have an
activity against P. acnes lipase activity are myricetin (IC50: 0.38 mM) and quercetin (IC50: 0.42 mM)13. But robinetin
which also belongs to the flavonol group does not inhibit but accelerates the lipase activity. Some of the reported
flavonols also has whitening activity14. It means that further research is needed to identify the active component
from F12. Distinct from fraction F12, fraction F1.2 had a carbonyl group which was not found in F12. Based on this
result the component in F1.2 could be flavonoid. Phytochemical test analysis showed that F1.2 consisted of auron.