The diethyl
ether fractions were dried under O2-free N2 and the
carotenoids and chlorophylls were dissolved in 1 ml ethyl
acetate. Chlorophylls and carotenoids were quantified by
measuring their absorbance at 480 nm, 648 nm, and 666 nm
in chloroform, using a Jasco V-530 UV/Vis spectrophotometer
(Jasco, Tokyo, Japan) and the pigment concentrations
calculated using the equation described by Wellburn (1994).
Flavonoids were extracted from 30 mg DW ground leaf
tissue as described above.