After the incubation, the mixture was centrifuged at 15,000g for 10min and the supernatant was transferred into a new test tube (cellwall fraction). For the analysis of cytoplasmic proteins, the pellet was dissolved in 10mMTris-HCl (pH8.0) and protease inhibitor cocktail and sonicated. After centrifugation at 15,000g for 10min, cytoplasmic soluble proteins were recovered (cytoplasmic fraction)