A—Maximum allowed number of markers with unbalanced peaks. B—Maximum
allowed number of peaks for each marker. C—Number of markers that
must contain peaks. D—Number of markers that must have peak heights above
a defined percentage of the threshold value. Class 1 includes complete profiles,
class 9 blanks and class 10 problematic profiles, e.g. mixtures. For a sample to
be automatically accepted by ForumDNA LIMS, the sum of the quality classes
for two analyses must be five or less. A marker is considered to have unbalanced
peaks if the height of the smaller peak is less than 50% of the height of the larger
peak. The threshold values (in rfu) are marker specific and are updated after
validation of each new batch of AmpF‘STR1 SGM Plus1 PCR mix. The
thresholds typically range from 150 to 250 rfu for heterozygotes, and from 300
to 500 rfu for homozygotes.