Results, Discussion and Conclusion: As a results of this study, 7 pilot research laboratories a case study at KMUTT-BKT. The safety checklist is used to find the hazard in laboratory in 8 areas that are (1) Working Area Setting (2) Electric System (3) Facilities Care (4) Ventilation (5) Fume Hood (6) Chemical and Waste Storage (7) MSDS (8) Safety Preparation. The result of safety inspection in laboratory found the first three hazards preventive measure that should be done are (1) Clear any obstructions within the operating room. (2) Install electrical protective equipment and should be removed any obstructions around electrical panel (3) Prepare the personal protective equipment properly for and should be inspect it's integrity always as shown in Table1and2. And, the result of Biosafety inspection found the “Bacillus subtilis wild type/GMO” in laboratory that high stability of the infection (spore forming). So, Operator should be work in only laminar flow cabinet. The hazard preventive measures will be applies and maintain to all laboratories in KMUTT later.