One in one dilution method was used with
slight modification. 10mls of fresh water extract was
made from 10mls each of sterile distilled water inside
series of sterile distilled water inside series of sterile
25mls bottles (a set of 8 plates) and war allowed to
solidify. The plates were then seeded with the isolates
by after which they were incubated at 370C for 24
hours.