Statistical analyses were performed using Genstat software (Genstat, 14th Edition,VSN International LTD, UK). All ten treatments were analysed in a randomized block with muscle as a block in a one- way analysis of variance (ANOVA). In a separate analysis, treatment time and ultrasonic intensity were set as factors in a two – way ANOVA to assess differences between ultrasonic treatments, excluding the control. Where a factor was significant means were compared using F protected least significant difference test (FLSD) at the 0.05 level. The results of the two – way ANOVA are presented only where a significant difference from the control was indicated by the one – way ANOVA.