The most potent cytotoxic agent against 5RP7 cell line was
found as compound 2 (IC50 ¼ 5.83 ± 1.04 mg/mL) followed by
compounds 10 (IC50 ¼ 12.33 ± 0.58 mg/mL) and 4
(IC50 ¼ 13.33 ± 1.53 mg/mL) when compared with mitoxantrone
(IC50 ¼ 0.73 ± 0.06 mg/mL). This outcome showed that p-nitro, 2,5-
dimethoxy and p-methoxy substituents increased cytotoxic activity
against 5RP7 cell line. On the other hand, compound 5 exhibited
low anticancer activity against 5RP7 cell line with an IC50 value of
68.33 ± 22.55 mg/mL.