Other methods of analysis
The extensive literature survey showed that, there are several methods which can be used for assaying amoxicillin in drug substances, formulation products and biological fluids. Literature survey revealed Ultraviolet spectroscopy to assay amoxicillin in formulated products. Ultraviolet of a derivative i.e. degradation of amoxicillin at pH 5.2 in the presence of cupric ion gives a product absorb at 320 nm, measurement of which was used as official assay method in British Pharmacopoeia, 1973. Ultraviolet of a derivative method can also be used to assay amoxicillin in urine, but may be interfered by penicilloic acid and other metabolites25.